Technology
One cavity, four sensitive measurements
How It Works
The sample to be analyzed sits inside a proprietary, high-albedo diffusing cavity. Laser light is scattered randomly hundreds to thousands of times before it escapes, stretching the effective optical path from centimeters to tens of meters. The same cavity, the same sample, and the same moment yield four readings at once: particle size (DLS), chemical structure (Raman), nucleic-acid/protein concentration (fluorescence), and protein concentration (UV-Vis absorption).
Why Cavity Amplification Matters
A conventional light scattering instrument reads a single pass of light through the sample. RIPPLE's diffusing cavity has demonstrated a published, peer-reviewed signal amplification of 100–1,700× over single-pass methods. This provides the basis for reaching more dilute, more rare, and more chemically detailed signals than earlier instruments could. The cavity's shape also ensures that light is scattered stochastically at all times and in all circumstances, meaning the set-up requires no alignment nor sample preparation.
Publications
- Theoretical predictions for ultrasensitive sensing in three-dimensional stochastic interferometry - Physical Review Research.
- Cavity Amplified Speckle Spectroscopy expands light scattering techniques to transparent and miniature samples - ACS Nano.
- 3D stochastic interferometry detects picometric fluctuations of an optical volume - Nature Communications Physics.
- Stochastic light concentration from 3D to 2D reveals ultraweak chemi- and bioluminescence - Scientific Reports.
- Detectivity optimization to measure ultraweak light fluxes with an EM-CCD as binary photon counter array - Scientific Reports.
- Marker-free protein study by amplified light scattering - Proceedings of SPIE.
- Random dynamic interferometer: cavity amplified speckle spectroscopy using a highly symmetric coherent field created inside a closed Lambertian optical cavity - Proceedings of SPIE.
- Protein Conformational Dynamics Probed By Correlation Spectroscopy of Multiply Scattered Light - Biophysical Journal.
Development Status
The RIPPLE Technology is still in an early stage but has been demonstrated in a lab-based benchtop prototype. Application-specific performance validations are currently ongoing.